Virgin Sister Defloration

[Yukiyanagi] Cry-Baby Sister (COMIC MEGAPLUS 2006-01) [English] [Yoroshii]

Kimi wa Seishunki - Dream in Adolescence | 思春期少女

Sisters ~Natsu no Saigo no Hi [eng][eichi]

Papakatsu Shimai. Kairakuzuke no Nakayoshi Shimaidon. | Sugar Baby Sisters. Getting Along and Drowning in Pleasure Fucking Two Sisters at the Same Time

[Rouge Not Blue (Utata)] Rouge Not Blue C's ~Twin-Tail Sisters~ (Gunslinger Girl, Sister Princess)

[Takeda Hiromitsu] Sister Breeder Ch. 1-2 [English]

[Fuetakishi] Kirei de Yarashii Oneesan wa, Suki Desuka? | Do You Like My Beautiful, Horny Older Sister? (COMIC Megastore 2011-05) [English] [Zenigeba + Ero Manga Girls]

Shojo no Mamade wa Irarenai | I Can't Stand Being a Virgin Girl!

[Oyster] Sister (Shoujo Jigoku) [English] =LWB=

SISUANA - sister hole

[Jyaco] Zettai Kengen Sisters! | Absolute Authority Sisters [English] {doujin-moe.us + YQII}

Imouto wa Boku no Ayatsuri Figure | My Sister Is My Puppet

Ecchi na Sister wa Okirai desu ka? | Do You Hate Lewd Sisters?

[Tsukako] Hatsukoi Party - Pure virgin for you... | 初戀派對 [Chinese] [Decensored] [Digital]

(C83) [Nikopondo (Aoyama Reo)] Sweet Little Sister [English] [Hayama_Kotono]

[Horitomo] Imouto wa Sukyura-tsu Musume | My Younger Sister Is A Scylla (COMIC Unreal 2012-06 Vol. 37) [English] =TV= [Decensored] [Digital]

Aku no Onna Shuryou to Doutei Kouseiin | Evil Leader and the Virgin Members =TLL + CW=

Boku to senpai to himitsu no koto。VIRGIN FLIGHT:06

Virgin Snow

[Miyano Kintarou] Hishojo List | Non-Virgin List 1-3 [English] {darknight} [Digital]

[Tsutsumi Akari] Ane Ippai no Ai wo | Full of Sisterly Love [English]

[Kisaragi Gunma] Giri Giri Sisters [English] [SaHa] [Decensored]

[Saegusa Kohaku] KURO-SiS -Black Sisters- [Chinese] [無邪氣漢化組]

[Gunma Kisaragi] Sister Syndrome [English] (Uncensored)

Sayonara Extra Virgin

[Gorgeous Takarada] Imouto Gokoro. - Sister's Heart. [English] [SaHa]

Boku no Onee-chan to Tomodachi wo Nemurasete Osottemitara Kaeriuchi ni Atta | The Tables were Turned when I tried to Rape my Sister and her Friends while they were Asleep

Sotsugyou Lost Virgin

[7cm (Nase)] Sugu no Shojo wa Nido Oishii | My Sister’s Virginity is Twice Delicious (Sword Art Online) [English] [EHCOVE] [Digital]

[Mokusei Zaijuu] A Virgin's Netorare Rape and Despair... ~To Tokyo Edition~ (COMIC Maihime Musou Act. 05) [English] =LWB=

[Yuuki Homura] Onee-chan! Tengoku | Sister Paradise Ch. 1-9 [English] [Lazarus H]

[Ohtomo Takuji] Gokkun Shojo - Drinking Virgin [English] [biribiri]

Bitch Gal to Shojo Sensei ni Hokenshitsu de Shiboritorareru. | Getting Wrung Out in the Infirmary by a Bitch Gal and a Virgin Nurse

[Iroito] Meshimase Imouto Gal à la mode | Experience the Younger Sister Gyaru à La Mode (COMIC ExE 06) [English] [N04h] [Digital]

[Shinobu Tanei] Gimai Shojo Gensou | Little Stepsister Fantasy Virgin Ch. 1-4 [English] {Tadanohito}

(C88) [40010 1-GO (40010Prototype)] Closest Sister (To LOVE-Ru) [Chinese] [脸肿汉化组]

[Nanase Mizuho] Ane Otouto Ane | Older Sister Little Brother Older Sister [English] {biribiri} [Decensored]

[clesta (Cle Masahiro)] CL-orc 01 Ane Zanmai - Three sister's harem [English] [Digital]

[Shinobu Tanei] Little Stepsister Love Space Ch. 1-6 [English] {Tadanohito}

Getting wrung out tenderly by holy maiden big sisters | Seijo Onee-chans to Amayakashi Nukinuki Seikatsu

[Yuuki Homura] Onee-chan! Tengoku | Sister Paradise Ch. 1-8 [English] [Lazarus H]

Chishojo Fuuki Iin no Minna ni Ienai Inbi na Onegai | The Perverted Virgin Public Morals Committee Member’s Secret Naughty Request

(C97) [Azure (Kagono Tori)] Ani ga Lolicon datta no de Imouto wa Doutei o Ubau koto ni shita | Since Big Brother is a Lolicon his Little Sister Decided to Seize his Virginity [English] {Mistvern}

[Akao, Anaran] Konomi ja Nai kedo ~Mukatsuku Ane to Aishou Batsugun Ecchi | She's Not My Type But ~Amazing Sex Chemistry With My Annoying Older Sister~ 1 [KenGotTheLexGs]

(C88) [ACIDSEA (Asuma Omi)] SWEET SCARLET SISTER (Fire Emblem if) [English]

[Maeshima Ryou] Virgin Bitch (COMIC LO 2013-05) [English] [Facedesk]

[Ooishi Kou] Ore to Imouto to Sono Kareshi no Sankaku Kankei | The Love Triangle Between Me, My Little Sister and Her Boyfriend (Nyotaika Ryoujoku!! III) [English] [desudesu] [Digital]

[Tamaki] Kanrinin san no Douteigui | Caretaker's Virgin Meal (COMIC ANGEL Club 2013-01) [English] =TV=

[Sumino Yuji] Bing! kang! Virgin!

I Bought Myself A Virgin

(CR30) [HarthNir (Misakura Nankotsu)] Binzume Sisters 2 (Sister Princess)

(C60) [HarthNir (Misakura Nankotsu)] Binzume Sisters 1-B (Guilty Gear, Sister Princess)

[Michiking] Ane Taiken Jogakuryou 1-11 | Older Sister Experience - The Girls' Dormitory [English] [Yuzuru Katsuragi] [Digital]

[Miyano Kintarou] Hishoujo List - The Non-Virgin List [Digital]

[Maka Fushigi] Tomodachi no Imouto. | My Friend's Little Sister (Gekisatsu! JS Collection) [English] [Jiko-tan]

(C88) [40010 1-GO (40010Prototype)] Closest Sister (To LOVE-Ru) [English] [PSYN] [Decensored]

[Mizuyoukan] My Little Sister ~Hitomi~ (Hakudaku Index Chapter 3) [ENG] [Yoroshii]

Imouto no Kareshi ga Kawaii no de | My Sister's Boyfriend is so cute that I...

[Labomagi! (Takeda Aranobu)] Chishojo Fuuki Iin no Minna ni Ienai Inbi na Onegai | The Perverted Virgin Public Morals Committee Member's Secret Naughty Request [English] [2d-market.com] [Decensored] [Digital]

[Agata] Natsu no Owari ni Ijiwaru Nee-chan - My mean elder sister at the end of summer. (Manga Bangaichi 2015-03) [English] [desudesu]